Skin Care Research: Ingredients to Avoid

Effect of benzoyl peroxide on antioxidant status, NF-κB activity and interleukin-1α gene expression

Benzoyl peroxide (BP) is used as a topical treatment for acne. Besides its anti-bacterial activity, the exact molecular mechanisms underlying its mode of action are not fully understood. In the current study, the effects of BP on cell viability, antioxidant status and, IL-1 and IL-8 gene expression were investigated in HaCaT keratinocytes. Keratinocytes incubated for 24 h with BP exhibited a dose-dependent cytotoxicity at concentrations above 250 μM. Furthermore, in the presence of 300 μM BP about 50% of the cellular vitamin E was depleted within the first 30 min. The intracellular ratio of oxidized to reduced glutathione (GSSG/GSH) was increased significantly starting 6 h after BP treatments indicating that BP reacts rapidly with targets in the cell membrane and more slowly with those in the cytosol. NF-κB transactivation was not significantly affected by BP. However, BP treatment of HaCaT keratinocytes resulted in a dose-dependent increase in IL-1α gene expression whereas no changes in IL-8 mRNA levels were observed. These results demonstrate that BP induces an inflammatory reaction mediated by oxidative stress by a pathway independent of the redox-sensitive transcription factor NF-κB.

Source: http://www.sciencedirect.com/science/article/pii/S0300483X01004309

Back to Skin Care Research

Email Signup
Receive exclusive offers, news and research.

Free ShippingShop with confidence
30 day money back guarantee
Free ShippingFree shipping on all US orders
and International orders over $250
  • Follow MVOrganics on Twitter
  • Friend MVOrganics on Facebook
  • MVOrganics on Pinterest
  • © 2013 Marie Veronique Organics. All rights reserved.
  • Awards and Recognition for MVO

Add to your MVO Wishlist